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Identification of yeasts from interspecific varieties of grapes
Sadel, Peter ; Omelková, Jiřina (referee) ; Vránová, Dana (advisor)
The main goal of my diploma thesis was to identify and characterize yeasts from must Hibernal and also collection yeasts by using methods called RFLP-PCR (Restriction Fragment Length Polymorphism - Polymerase Chain Reaction). Theory was the first part of my diploma thesis which dealt with wine, yeasts and molecular methods. Theory section was followed by experimental section divided into two parts. The main goal of the first part was to characterize and identify yeasts from must Hibernal by using PCR and RFLP-PCR methods. In the samples there were found yeasts Saccharomyces and Pichia. The second experimental part of my diploma thesis had a goal to extend the database of new yeasts using the same methods mentioned in the first part of experimental section.
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Selection of appropriate indigenous yeasts for wine production
Krátká, Veronika ; Babák, Libor (referee) ; Vránová, Dana (advisor)
The aim of this diploma thesis was the selection of appropriate indigenous yeasts for wine production. Tested yeasts were isolated from the grapes from the winery Maňák Žádovice. The yeasts isolated within theses in 2009 – 2012 have been also tested, and commercial wine yeast have been tested for comparison. In the theoretical part the focus is on the technology of wine, in particular fermentation. The work is also focused on yeasts metabolism and taxonomy. There was described the principle of PCR-RFLP, and methods used to characterize the properties of isolated yeasts. In the experimental part was made isolation of yeasts, theirs identification using PCR-RFLP and to select the most suitable yeast in wine making proces were performed physiological tests.
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Laktózová intolerance: výskyt ve světové populaci a možnosti její diagnostiky
CHÁNOVÁ, Jiřina
The aim of the theoretical part of this bachelors thesis was to give the summary of current knowledge of the topic 'lactose intolerance'. There are being characterized the cause of lactose intolerance, the global prevalence, possibilities of its diagnosis and of its treatment. The practical part deals with the methodology that I used for the detection of C/T-13910 and G/A-22018 polymorphism. In the genetic laboratory were tested 34 individuals for the lactose intolerance with method RFLP-PCR.
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Significance of detection of the ApoE gene and MTHFR gene polymorphisms for osteoporosis.
ŠILHOVÁ, Markéta
Osteoporosis is the sceletal metabolic disease characterized by changes of bone tissue and disfunction of mechanic bone resistance which can lead to numerous bone fractures. Osteoporosis is frequently diagnosed in patients older than 60 years, also advanced age patients are not an exception for this disease. The most frequently affected group of patients is postmenopausal women. Prevalence of osteoporosis still rises. Nowadays more than 200 millions of people suffer from osteoporosis and one million of patients from Czech republic. Osteoporosis have became a serious complication affecting not only medical but also an economic field. Based on these findings, osteoporosis should be considered as an important topic even from the perspective of genetic risk factors. The aim of my bachelor thesis is to write a specialized research focused on introduced topic, summarize accessible information about osteoporosis, including diagnosis and genetic predisposition. I have focused especially on genes ApoE and MTHFR677. ApoE plays an important role in sceletal homeostasis. The highest risk for osteoporosis development is the presence of alel 4 gene for alipoprotein E. Gen MTHFR677 is mostly associated with bone mineral density located in femoral neck. An experimental part of bachelor thesis was based on laboratory practice in molecular biology field inluding isolation of DNA from peripheral blood, PCR technique, detection of PCR products by gel agarose electrophoresis, analysis of ApoE gel by MyTaq Red DNA Polymerase kit and 2x PCRBIO HS Taq Mix, analysis of MTHFR677 gene by MyTaq Red DNA Polymerase kit. Analysis of ApoE gene is based on ARMS PCR method, on the other hand analysis of MTHFR677 is based on RFLP PCR method. I have performed the laboratory practice within the Laboratory of genetics GENLABS in České Budějovice.
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Application of the method RFLP PCR , PCR ARMS and reverse hybridization for detecting the most common thrombophilic mutations in the Czech population
MATĚJKOVÁ, Markéta
Recently the term thrombophilia is becoming a current topic. It is an increased precondition for the creation of tromboses and the following complications as the result of increased blood coagulation which could be very serious or even fatal. A timely diagnosis of thrombophilia mutations helps patients to avoid many critical situations (e.g. in case of operations, pregnancy etc.). In these days there are at least 30 genetic laboratories in the Czech Republic which research thrombophilia mutations. Unfortunately this medical examination is covered by insurance companies just in case of indicated symptoms which are under strict criteria. If someone is interested in a medical examination and a doctor does not indicate him with the symptoms, they are forced to pay the examination on their own. There are a few insurance companies which contribute with some amount for these examinations as a part of their preventive programmes (e.g. Česká průmyslová zdravotní pojišťovna, Zdravotní pojišťovna ministerstva vnitra ČR or Zdravotní pojišťovnu Revírní bratrská pokladna). The aim of my bachelor thesis was a practical mastery RFLP-PCR method, PCR ARMS method and a reverse hybridization on strips to detect the most common mutations of thrombophilia in the population of the Czech Republic and deviloving on the issue. In the theoretical part, I focus on a description of the most important thrombophilic mutations in the Czech population and their risks. I also describe particular genetic methods by which each of thrombophilia mutations is being examined in genetic laboratories. My own results of the examination of four major thrombophilia mutations and polymorphs are shown in the practical part. These are the Leiden mutation, prothrombin mutation, MTHFR 1298 and MTHFR 677 polymorphs. For my examinations I used these genetic methods: RFLP-PCR method, PCR ARMS method, reverse hybridization on strips. I made all these methods in the genetic laboratory Genlabs in České Budějovice. For the examinations I used DNA samples of my family.
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Identification of yeasts from interspecific varieties of grapes
Sadel, Peter ; Omelková, Jiřina (referee) ; Vránová, Dana (advisor)
The main goal of my diploma thesis was to identify and characterize yeasts from must Hibernal and also collection yeasts by using methods called RFLP-PCR (Restriction Fragment Length Polymorphism - Polymerase Chain Reaction). Theory was the first part of my diploma thesis which dealt with wine, yeasts and molecular methods. Theory section was followed by experimental section divided into two parts. The main goal of the first part was to characterize and identify yeasts from must Hibernal by using PCR and RFLP-PCR methods. In the samples there were found yeasts Saccharomyces and Pichia. The second experimental part of my diploma thesis had a goal to extend the database of new yeasts using the same methods mentioned in the first part of experimental section.
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Selection of appropriate indigenous yeasts for wine production
Krátká, Veronika ; Babák, Libor (referee) ; Vránová, Dana (advisor)
The aim of this diploma thesis was the selection of appropriate indigenous yeasts for wine production. Tested yeasts were isolated from the grapes from the winery Maňák Žádovice. The yeasts isolated within theses in 2009 – 2012 have been also tested, and commercial wine yeast have been tested for comparison. In the theoretical part the focus is on the technology of wine, in particular fermentation. The work is also focused on yeasts metabolism and taxonomy. There was described the principle of PCR-RFLP, and methods used to characterize the properties of isolated yeasts. In the experimental part was made isolation of yeasts, theirs identification using PCR-RFLP and to select the most suitable yeast in wine making proces were performed physiological tests.
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